期刊名称:CYTOMETRY PART A
期刊简介(About the journal)
投稿须知(Instructions to Authors)
编辑部信息(Editorial Board)
About the journal
Aims and Scope
Cytometry Part A embraces all aspects of analytical cytology and cytomics including flow cytometry, image cytometry, bead-based array analyses, slide-based array analyses, as well as other cell-based spectroscopic analyses. The research featured in the journal encompasses not only the development of the techniques and reagents needed to measure cell features and cellular constituents (molecules), but also investigations that primarily employ these techniques for characterization in order to provide an understanding of function and regulation in the context of the cell, organ, and organism. Cytometry publishes original research articles, in-depth reviews, rapid communications of new, novel "hot" topics, and technical innovation articles.
Instructions to Authors
Editorial Policy
Cytometry is the Official Journal of the International Society for Analytical Cytology. Clinical Cytometry is an Official Publication of the Clinical Cytometry Society and The International Society for Analytical Cytology
The scope of Cytometry embraces all aspects of analytical cytology, which is defined broadly as characterization and measurement of cells and cellular constitutents for biological, diagnostic, and therapeutic purposes. It includes components of cytochemistry, cytophysics, cell biology, molecular biology, physiology, pathology, image analysis, statistics, instrumentation, clinical laboratory practice, and other relevant subjects. Manuscripts may include new experimental results, descriptions of new methods or modifications of existing ones, or new interpretations of previously published data.
Manuscripts from all countries are invited but must be in English. Authors in Japan please note: Wiley-Japan can provide authors in Japan with a list of recommended services to check and improve the English in their papers BEFORE SUBMISSION. Please contact Masayo Kobayashi in the Wiley-Japan office by fax: 81 3 3556 9763 or E-mail: wileyjpn@mb.kom.ne.jp for more information.
Authors are not required to be members of the International Society for Analytical Cytology.
Review of Manuscripts An expert and anonymous reviewing process is important in assessing the scientific soundness of contributions to Cytometry and frequently helps authors to present their findings clearly and concisely. The Editorial Board of Cytometry selects reviewers, assists in review of manuscripts, makes recommendations concerning the suitability of manuscripts for publication, and advises the Editor on matters related to publication policy. All manuscripts will be evaluated independently by two reviewers. Reviewers are requested to submit written evaluations employing the following criteria:
1. Does the subject matter fall within the scope of Cytometry? 2. Does the paper describe original findings and add significantly to knowledge in its field? 3. Are the aims, results, and discussion presented clearly and concisely? 4. Are the experimental methods generally acceptable and are they described in sufficient detail to permit repetition of the work? 5. Is the documentation of the findings adequate? 6. Are the illustrations appropriate and of acceptable quality? 7. Are quantitative results statistically supported? 8. Is the list of references current and complete?
The Editor-in-Chief, on the basis of recommendations from the reviewers and the Editorial Board, may accept the manuscript, decline it, return it for revision, or send it to a third reviewer to referee differences of opinion between the initial reviewers.
Forms of Publication. Cytometry publishes five types of contributed papers:
Articles are full-length reports of original research in all fields of analytical cytology. The investigation may contribute to the development, evaluation, or application of cytometric methods and their use in biology and medicine.
Reviews are comprehensive appraisals of current cytometric issues and advances. They are subject to the same review process as other papers. Reviews are welcomed, but authors should consult the Editor-in-Chief about the suitability of a proposed Review before its preparation.
Technical Notes briefly describe improvements or helpful modifications in procedures for cytometric and related techniques. The Technical Note should include a brief statement of purpose, an adequately detailed description of the procedure, a statement of expected results, and references to pertinent literature. Observations based on the application of the method also may be included.
Rapid Communications are original articles of such timeliness and interest that they merit priority processing. Authors should submit their manuscript with a letter explaining their reasons for requesting rapid processing. If the Editor agrees with the request, the manuscript will receive an expedited review and, when accepted, priority for publication; otherwise the manuscript will be processed as a regular submission.
Letters to the Editor provide a forum for communication of opinion, interpretation, and new information on scientific/political matters. Letters are judged on appropriateness of the subject and interest to the readers. They should be concise and to the point.
Length of Manuscripts and Page Charges
Authors are reminded that brevity is a virtue and contributes to the clarity of their contribution. Normally, articles will be limited to about 5,000 words and a combined total of six figures and tables. Authors will be asked to shorten excessively verbose manuscripts. Authors of articles that exceed eight printed pages will be charged $150 per excess page. Prior to publication, the Editor may waive this charge for authors who specifically request exemption and who provide appropriate reasons to justify their request.
Manuscript Submission
Authors are strongly encouraged to submit their manuscripts online at http://cyto-wiley.manuscriptcentral.com/.
Authors are encouraged to include the names and addresses of persons outside their own institutions who might be qualified to serve as reviewers. Authors can request their manuscript not be reviewed by reviewers whose opinion, they believe, may be unfavorably biased. Authors may also request their manuscript be transmitted to specific members of the Editorial Board.
Copyright. Submission of a manuscript implies that it has been approved by all the named authors, that it reports unpublished work, and that it is not under consideration for publication elsewhere. If the manuscript is accepted for publication, it will become the sole property of the publisher and will be copyrighted in the name of the publisher. The manuscript, in whole or part, must not be published elsewhere in either English or another language.
Manuscript Preparation
Manuscript Organization
All articles should be double-spaced throughout, with each page numbered consecutively. Begin each section, described below, on a new page.
- Title Page:
- The opening page must contain: a) complete title (be succinct and informative, avoid subtitles); b) name and affiliations of each author (specific to the department level); c) a running headline, not to exceed 45 characters in length, including spaces; d) complete contact information -- the corresponding author's mailing address, phone and fax numbers and e-mail address; e) credits for research support as a footnote to the title; and f) any important miscellaneous information, e.g., that the research is based on a conference presentation, also as a footnote to the title.
- Abstract and Key terms:
- Authors are required to provide a structured abstract. The abstract, which should not exceed 200 words, should consist of four paragraphs, labeled: Background, Methods, Results, Conclusions.They should describe, respectively, the reason for the study, how the study was performed, the most important results, and what is concluded from the results. Below the abstract, provide three to ten key words or short phrases for indexing. The subject under investigation and technologies or methods employed must be included. The Medical Subject Headings List from Index Medicus should be used whenever possible.
- Introduction:
- State the aim of the work and the problem that stimulated it. Briefly summarize relevant published investigations.
- Materials and Methods:
- Present in sufficient detail to permit the work to be repeated by other investigators.
- Results:
- Present results concisely, avoiding redundant use of both tables and figures to illustrate the same data set.
- Discussion:
- Limit to interpretation of results, with a minimum of recapitulation of findings.
- References:
- Cite references in text and list at the end of the article. Follow the instructions for Literature Cited, below.
- Figures, Tables, and their Legends:
- Illustrations, tables and legends must be numbered consecutively and cited sequentially in text; design these to be self-explanatory without reference to the text.
General Rules and Style
- Spelling:
- Cytometry uses Webster's New Collegiate Dictionary as its standard for spelling.
- Style:
- For information on style, abbreviations, nomenclature, and the preparation of copy, authors are referred to the Council of Biology Editors (CBE) Style Manual 6th edition, available from the Council of Biology Editors, Inc., 11 South La Salle Street, Chicago, IL 60603-1210. Abbreviations and acronyms are spelled out at their first use and abbreviated in parentheses immediately thereafter.
- Units:
- The units for all Measurements must conform to the International System of Units (SI). If a measurement is made in other units, then the SI equivalent should be given in parentheses.
- Products and Companies:
- If authors refer to specific products, they must identify the company by citing, in parentheses, the company's name, city, and state or country.
- Literature Cited:
Wiley's Journal Styles Are Now in EndNote EndNote is a software product that we recommend to our journal authors to help simplify and streamline the research process. Using EndNote's bibliographic management tools, you can search bibliographic databases, build and organize your reference collection, and then instantly output your bibliography in any Wiley journal style. Download Reference Style for this Journal: If you already use EndNote, you can download the reference style for this journal. How to Order: To learn more about EndNote, or to purchase your own copy, click here. Technical Support: If you need assistance using EndNote, contact endnote@isiresearchsoft.com, or visit www.endnote.com/support.
- References may be made only to published works and papers in press. Work in progress, unpublished experiments, and personal communications are specifically excluded from the reference list but may be acknowledged in parentheses in the text. Citation of references in text must use the Citation-Sequence format in which the reference number is entered in parentheses.
References must be listed sequentially at the end of the maunscript. Abbreviations of journal names must conform to those used in Index Medicus. Authors are responsible for the accuracy of the references. The following formats must be used:
- Journal Article
- 1. Hüller R, Glossner E, Schuab S, Weingärtner J, Kachel V. The macro flow planktometer: a new device for volume and fluorescence analysis in macro plankton including triggered video imaging in flow. Cytometry 1994;17:109-118.
- Article in a book or comparable publication
- 2. Gary JW, Cram LS. Flow karyotyping and chromosome sorting. In: Melamed MR, Lindmo T, Medelsohn ML, editors. Flow cytometry and sorting. 2nd ed. New York: John Wiley & Sons Inc.; 1990. p 503-529.
- Books
- 3. Givan AL. Flow cytometry: first principles. New York: John Wiley & Sons Inc.;1993. 1223 p.
Editorial Office Requests for copies of the Editorial Policy and Instructions to Contributors, requests for clarification of specific issues, manuscripts submitted for publication, and all other communications with the Editorial Office should be addressed to the Editor-in-Chief:
Charles L. Goolsby, Editor-in-Chief Northwestern University Medical School Department of Pathology 303 East Chicago Avenue, Ward Building 6-204 Chicago, IL 60611-3008 Telephone: 312-503-1847 Telefax: 312-503-1848 E-mail: cytometry@nwu.edu
European contributors may address their requests to the European Editor:
Janos Szollosi, Ph.D. Department of Biophysics and Cell Biology Medical University, School of Debreceen, P.O. Box 39 Nagyer dei krt, 98 Debreceen, Hungary, H-4012 E-mail: szollo@jaguar.dote.hu
Bioimaging contributors may address their requests to the Bioimaging Editor:
Stephen Lockett NCI-Frederick Boyle St., Bldg. 1050 Frederick, MD 21702 Telephone: 301-846-5515 E-mail: sjlockett@lbl.gov
Reprints Reprints are supplied by the publisher at a cost based on the number of pages in the printed article and the number of reprints ordered, as shown on the order blank mailed with the proofs. Wiley is pleased to extend a 50% discount on reprint orders to ISAC and CCS members.
Disk Submission Instructions
Please return your final, revised manuscript on disk as well as hard copy. The hard copy must match the disk.
The Journal strongly encourages authors to deliver the final, revised version of their accepted manuscripts (text, tables, and, if possible, illustrations) on disk. Given the near-universal use of computer word-processing for manuscript preparation, we anticipate that providing a disk will be convenient for you, and it carries the added advantages of maintaining the integrity of your keystrokes and expediting typesetting. Please return the disk submission slip below with your manuscript and labeled disk(s).
Guidelines for Electronic Submission
Text Storage medium. 3-1/2" high-density disk in IBM MS-DOS, Windows, or Macintosh format.
Software and format. Microsoft Word 6.0 is preferred, although manuscripts prepared with any other microcomputer word processor are acceptable. Refrain from complex formatting; the Publisher will style your manuscript according to the Journal design specifications. Do not use desktop publishing software such as Aldus PageMaker or Quark XPress. If you prepared your manuscript with one of these programs, export the text to a word processing format. Please make sure your word processing program's "fast save" feature is turned off. Please do not deliver files that contain hidden text: for example, do not use your word processor's automated features to create footnotes or reference lists.
File names. Submit the text and tables of each manuscript as a single file. Name each file with your last name (up to eight letters). Text files should be given the three-letter extension that identifies the file format. Macintosh users should maintain the MS-DOS "eight dot three" file-naming convention.
Labels. Label all disks with your name, the file name, and the word processing program and version used.
Illustrations All print reproduction requires files for full color images to be in a CMYK color space (not RGB). If possible, ICC or ColorSync profiles of your output device should accompany all digital image submissions.
Storage medium. Submit as separate files from text files, on separate disks or cartridges. If feasible, full color files should be submitted on separate disks from other image files. 3-1/2" high-density disks, CD, Iomega Zip, and 5 1/4" 44- or 88-MB SyQuest cartridges can be submitted. At authors' request, cartridges and disks will be returned after publication.
Software and format. All illustration files should be in TIFF or EPS (with preview) formats. Do not submit native application formats.
Resolution. Journal quality reproduction will require greyscale and color files at resolutions yielding approximately 300 ppi. Bitmapped line art should be submitted at resolutions yielding 600-1200 ppi. These resolutions refer to the output size of the file; if you anticipate that your images will be enlarged or reduced, resolutions should be adjusted accordingly.
File names. Illustration files should be given the 2- or 3-letter extension that identifies the file format used (i.e., .tif, .eps).
Labels. Label all disks and cartridges with your name, the file names, formats, and compression schemes (if any) used. Hard copy output must accompany all files.
Standard Abbreviations for Cytometry
2D two-dimensional
2-ME 2-mercaptoethanol
3D three-dimensional
7-AAD aminoactinomycin D
a.u. arbitrary unit
Ab antibody
ABC antibody binding capacity
ac alternating current
ADC analog to digital converter
Ag antigen
AIDS acquired immune deficiency syndrome
ALL acute lymphoblastic leukemia
AMCA 7-amino-4-methylcoumarin-3-acetic acid
AML acute myeloblastic leukemia
ANN artificial neural network
APC allophycocyanin
Ara-C cytosine arbinoside
ATP adenosine triphosphate: ADP, AMP, GDP, GMP, GTP, CTP, UDP
BCECF 2,7-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein
bp base pair
BrdU bromodeoxyuridine
BSA bovine serum albumin
CD cluster of differentiation
CDKs cyclin dependent kinases
cDNA complementary DNA
CGH comparative genomic hybridization
CLL chronic lymphocytic leukemia
CML chronic myeloid cytic leukemia
CMV cytomegalovirus
ConA concanavalin A
cpm counts per minute
CSF colony-stimulating factor
CV coefficient of variation
DAB 3,3 diaminobenzidin
DAPI 4,6-diamidino-2-phenylindole, dihydrochloride
DC dendritic cell
dc direct current
DMEM Dulbecco's minimum essential medium
DMSO dimethylsulfoxide
DNA deoxyribonucleic acid
DNase deoxyribonuclease
DPH 1,6-diphenyl-1,3,5-hexatriene
ds double stranded
EB ethidium bromide
EBV Epstein-Barr virus
EDTA ethylene-diaminetetraacetic acid
ELISA enzyme-linked immunosorbent assay
EM electron microscopy
ER endoplasmatic reticulum
EST expressed sequence tag
FACS fluorescence-activated cell sorting
FC(B)S fetal calf (bovine) serum
FCM flow cytometry
FDA fluorescein diacetate
FISH fluorescence in situ hybridization
FITC fluorescein isothiocyanate
FRET fluorescence resonance energy transfer
FSC forward angle scattered light: FLS
FWHM full width at half maximum
g gravity
G-CSF granulocyte colony-stimulating factor
GFP green fluorescent protein
GM-CSF granulocyte-macrophage colony-stimulating factor
GSH glutathione
Hb hemoglobin
HbF fetal hemoglobin
HBSS Hank's balanced salt solution
HEPES N-2-hydroxyethelpiperazine-N2 ethansulphonic acid
HIV human immunodeficiency virus
HLA human leukocyte antigen
HPLC high performance liquid chromatography
HSA human serum albumin
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i.m. intramuscular
i.p. intraperitoneal
i.v. intravenous
I/O input/output
IEF isoelectric focusing
IFN interferon
Ig immunoglobulin
IL interleukin
IMDM Iscove's modified Dulbecco's medium
IU international unit
kb kilobase
kDa kilodalton
LED light emitting diode
M molar
mAb monoclonal antibody
M-CSF macrophage CSF
MDR multidrug resistance
MESF molecules of equivalent soluble fluorochrome
MFI mean fluorescence intensity
MHC major histocompatibility complex
MNC mononuclear cell
mRNA messenger ribonucleic acid
MW molecular weight
n or No number
NK cell natural killer cell
NS not significant
OD optical density
P probability
PAGE polyacrylamide gel electrophoresis
PBL peripheral blood lymphocyte
PBMC peripheral blood mononuclear cells
PBS phosphate-buffered saline
PCR polymerase chain reaction
PE phycoerythrin
PEG polyethylene glycol
PerCP peridinin chlorophyll protein
PHA phytohemagglutinin
PI propidium iodide
PKC protein kinase C
PMN polymorphonuclear leukocyte
PMT photomultiplier
PWM pokeweed mitogen
RAM random access memory
Rb retinoblastoma protein
RBC red blood cells
RFLP restriction fragment length polymorphism
RIA radioimmunoassay
RNA ribonucleic acid
rpm revolutions per minute
s.c. subcutaneous
SAv streptavidin
SBA soybean agglutinin
SCF stem cell factor, kit ligand, Steel factor
SCID severe combined immunodeficiency
SD standard deviation
SDS sodium dodecyl sulfate
SE standard error
SEM standard error of the mean
sIg surface immunoglobulin
ss single stranded
SSC side angle scattered light
SV40 Simian virus 40
t?BR>half-life, half-time
TCR T cell receptor
TdT terminal deoxynucleotidyl transferase
TNF tumor necrosis factor
TRITC tetramethyl rhodamine
TUNEL terminal dUTP nick end labeling
UV ultraviolet
vol volume
WBC white blood cells
WGA wheat germ agglutinin
YAC yeast artificial chromosome
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Editorial Board
E d i t o r - i n - C h i e f
Charles L. Goolsby, Ph.D. Northwestern University Medical School Department of Pathology Ward Building 6-204 303 East Chicago Avenue Chicago, IL 60611-3008 Telephone: 312-503-1847 Telefax: 312-503-1848 E-mail: cytometry@nwu.edu
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Reviews Editor
James Jacobberger Ralph Durand
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European Editor
Janos Szollosi
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Bioimaging Editor
Stephen Lockett
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A s s o c i a t e E d i t o r s
David Galbraith David Hedley James Jacobberger James Jett William Karpus Paul Smith Attila Tárnok Nicholas Terry Gunter Valet
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E d i t o r i a l B o a r d |
Nigel P. Carter Cambridge, United Kingdom
Kenneth Castleman League City, Texas
L. Scott Cram Los Alamos, New Mexico
Harry A. Crissman Los Alamos, New Mexico
Zbigniew Darzynkiewicz Hawthorne, New York
Caroline Dive Manchester, United Kingdom
Walter Giaretti Genoa, Italy
Jonathan Kagan Rockville, Maryland
Keiji Kawamoto Osaka, Japan
Ruth Knuechel-Clarke Regensburg, Germany
Awtar Krishan Miami, Florida
James Leary Galveston, Texas
John Lehman Albany, New York
Katharine A. Muirhead Malvern, Pennsylvania
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Jose-Enrique O'Connor Valencia, Spain
Michael G. Ormerod Reigate, United Kingdom
Maria Pallavicini San Francisco, California
Peter S. Rabinovitch Seattle, Washington
Frans C.S. Ramaekers Maastricht, The Netherlands
Mario Roederer Bethesda, Maryland
Kohsuke Sasaki Yamaguchi, Japan
Larry Sklar Albuquerque, New Mexico
Harald B. Steen Oslo, Norway
Hans Tanke Leiden, The Netherlands
Frank Traganos Hawthorne, New York
Yves Usson La Tronche, France
Lucas van Vliet Delft, The Netherlands
R. Allen White Houston, Texas
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E d i t o r i a l O f f i c e
Joy Holliday E-mail: cytometry@nwu.edu
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