期刊名称：PROTEIN SCIENCE
 期刊简介(About the journal)
投稿须知(Instructions to Authors)
编辑部信息(Editorial Board)
About the journal
Protein Science serves as an international forum for publishing original reports on proteins in the broadest sense. The Journal aims to unify this field by cutting across established disciplinary lines and focusing on “protein-centered?science.
Protein Science encompases the structure, function, and biochemical significance of proteins; their role in molecular and cell biology, genetics, and evolution; and their regulation and mechanisms of action. Representative topics include, but are not limited to, the structure of proteins and strategies of determining protein structure by chemical, biophysical, and recombinant methods; peptides; protein domains; protein folding and molecular dynamics; novel isolation procedures; enzyme action and regulation; interactions of proteins with nucleic acids, lipids, ligands, and other proteins; receptor-mediated signal transduction and other trans-membrane phenomena; the functions of proteins in replication, supramolecular assembly, immune reactions, development, and other biological processes; protein trafficking, synthesis, and sorting; and the recognition, localization and signaling of proteins. In addition to the investigative techniques already mentioned, the Journal will publish results of protein-centered work involving sequencing, modification, and mass spectrometry; cDNA, mutagenesis, and cloning; computational analysis; isolation and characterization; thermodynamics and hydrodynamics; kinetics; and equilibrium phenomena.
Protein Science publishes full-length original research papers as well as reviews and book reviews. Recollections (by invitation) are historical reviews that are intended to give an overview of how a particular topic in the science of proteins has developed. A special section, For the Record, features brief refereed reports of recent developments in the field. For example, this section may highlight a new protein sequence or report a new protein structure and/or function. Reviews (by invitation) are intended to familiarize the general reader with the current status and future trends of rapidly evolving topics of current interest.
Instructions to Authors
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- Table of Contents
- Overview
- Articles Published
- Copyediting and Page Proofs
- Page Charges
- Offprints
- Originality and Copyright
- Manuscript Submission and Review
- Paper Manuscript Preparation and Style
- Electronic Manuscript Preparation and Submission
- Electronic Edition
- Manuscript Elements and Order
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- Title page
- Abstract and keywords
- Abbreviations and symbols
- Introduction
- Results
- Discussion
- Materials and methods (Note: This section follows Discussion section!)
- Electronic Supplementary Material
- Acknowledgments
- References
- Tables
- Figure legends
- Figures (artwork); Electronic art submission
- Supplementary material to appear in the Electronic Edition
- Data Deposition Requirements: Protein and Nucleic Acid Coordinates
- Data Deposition Requirements: Protein and Nucleic Acid Sequences
- Checklist for Manuscript Submission and Electronic Supplementary Material
Protein Science serves as an international forum for publishing original reports on proteins in the broadest sense. The Journal aims to unify this field by cutting across established disciplinary lines and focusing on “protein-centered?science.
Protein Science encompasses the structure, function, and biochemical significance of proteins; their role in molecular and cell biology, genetics, and evolution; and their regulation and mechanisms of action. Representative topics include, but are not limited to, the structure of proteins and strategies of determining protein structure by chemical, biophysical, and recombinant methods; peptides; protein domains; protein folding and molecular dynamics; novel isolation procedures; enzyme action and regulation; interactions of proteins with nucleic acids, lipids, ligands, and other proteins; receptor-mediated signal transduction and other trans-membrane phenomena; the functions of proteins in replication, supramolecular assembly, immune reactions, development, and other biological processes; protein trafficking, synthesis, and sorting; and the recognition, localization, and signaling of proteins. In addition to the investigative techniques already mentioned, the Journal will publish results of protein-centered work involving sequencing, modification, and mass spectrometry; cDNA, mutagenesis, and cloning; computational analysis; isolation and characterization; thermodynamics and hydrodynamics; kinetics; and equilibrium phenomena. Reports on the crystallization of proteins must provide novel information of general interest. Examples of novel information may include new approaches to expression or isolation of proteins in a form amenable to crystallization, novel conditions for crystallization that may be employed for other proteins, or new information related to the function of the protein. Research articles on computational or theoretical aspects of protein structure and function must report results that can be (or have been) validated by experiments. There should be clear biological significance to the work, and the article should be of general interest to the Protein Science community. Novel approaches are encouraged; modifications of current algorithms without indication of significant improvements are not acceptable. Clear connections to significant problems related to protein structure and function must be demonstrated. Straightforward applications of generally available programs are not likely to receive strong editorial support. Please contact the Editorial Office or Associate Editors for clarification of appropriate subject matter.
A primary consideration in judging the suitability of a manuscript is its originality and timeliness, the lack of which will be grounds for rejection, even if the work is well done from a technical perspective. Because judgments of originality and interest are inherently subjective, this policy implies that the editors and reviewers reserve the prerogative to decline publication on these grounds.
Protein Science publishes full length original research papers as well as several other types of articles.
Full Length Papers must report new findings that lead to significant advances in our understanding of the proteins of interest. The investigation should be at a point at which the major objectives have been accomplished and the findings can be convincingly presented in a concise article. Only the definitive evidence required to support the authors?points should be presented; ancillary supporting data, if needed, should be deposited in the electronic supplement. Since Protein Science has contractual limitations on the number of pages it prints each year, it is very important that the articles be as concise as possible.
Accelerated Communications are intended to provide an outlet for brief papers of exceptional importance and timeliness. The criteria for acceptance will be considerably more stringent than for full papers, and there will be a limit of 2,500 words in the body of the manuscript plus a maximum of four figures, tables, or combination thereof. The work must be complete and stand on its own merits. Accelerated Communications will be reviewed by one of the Editors and a member of the Editorial Advisory Board; every effort will be made to reach a decision within three weeks. The decision will be Accept or Reject (except for minor corrections), and accepted articles will be published in the next available issue of the journal. Manuscripts that exceed the size limits will be returned without review. The format may be continuous prose or divided into the traditional sections. All other procedures will be according to the Instructions for Authors.
For the Record articles are intended to enable the publication of an interesting and important observation that should be in the literature. Articles reporting “one experiment?that are clearly part of a larger investigation are not appropriate. Similarly, success with a procedure that is not guaranteed to produce results (i.e., crystallization of a protein) is not appropriate unless the authors have employed novel approaches that will be applicable to other systems and thus are of interest to a broader audience. The information must stand on its own merits. Manuscripts submitted to “For the Record?should contain a short abstract and keywords, and introduction and discussion sections (separate or combined) for a maximum of three journal pages in length. A single illustration conveying essential information, such as a protein sequence, is encouraged.
Recollections (by invitation) are historical reviews that are intended to give an overview of how a particular topic in the science of proteins has developed. “Recollections?are intended to give an overview of how the understanding of an interesting topic in Protein Science developed, including protein structure, protein properties, and general or specific biological roles of proteins. A contribution may be based largely on personal participation in research or may give a perspective of how knowledge in an area developed. The anecdotal accounts are intended to give insight and background usually lacking in conventional scientific papers. They may have an autobiographical flavor.
Contributions can vary in length, with no specified format other than that a title, author’s name and affiliation, and a brief biographical sketch should be included. Usually figures and tables are not necessary but if included should have titles and legends. Inclusion of a few selected references is optional. One or more photographs may add interest. If a photograph has been previously published, written permission for its use must be included.
Reviews (by invitation) are intended to familiarize the general reader with the current status and future trends of rapidly evolving topics of current interest. Unsolicited Reviews will also be considered if preceded by a letter of inquiry stating the title, scope, and tentative outline of the proposed Review article. “Review?manuscripts may vary in length from three to five printed pages. They should follow the general format of original reports except that, as a rule, they do not include a Materials and methods section.
The Publisher reserves the right to copyedit manuscripts to conform to Protein Science journal style. The corresponding author will receive page proofs for proofreading. Proofs must be returned to the publisher within 48 hours. No rewriting of the final accepted manuscript is permitted at the proof stage. Excessive author alterations in proof may cause delays in publication and will be charged to the author.
Authors of accepted manuscripts will be assessed a page charge of US $35 per printed page for the first five pages and US $60 per page for each page over five. Authors will be billed at the time of proofing.
With the page proofs, the corresponding author will receive a form to order offprints and copies of the complete issue at special contributor rates. Orders received after issue printing require special print runs and are subject to reprint charges (quotes will be provided on a case-by-case basis by the publisher).
One condition of publication is that all manuscripts submitted to Protein Science have not been published previously nor are they under consideration for publication elsewhere. Electronic publication open to viewers by subscription or by unrestricted access to a World Wide Web site is considered prior publication with the exception of dissertations mounted in electronic format by the library system of the degree-granting institution. Papers involving multiple authorship are reviewed with the understanding that all authors have approved the final submitted manuscript and concur in its submission to Protein Science. Copyrights to all papers must be assigned to The Protein Society by a signed Copyright Status Form before the Editor will transmit the paper for publication.
Authors are not required to be members of The Protein Society.
Online manuscript submission. As of March 1, 2003, papers may be submitted by the author to our online manuscript submission system available at http://submit.proteinscience.org. Files that are compatible with our system include: Word, WordPerfect, and PDF (Portable Document File). Image and table file formats accepted are GIF, TIFF, EPS, and JPEG. For tables, the system accepts most word processing formats as well. Please note: The system does not accept Excel files at this time. Please paste tables from Excel into a Word document format. Supplemental files may be submitted in any format. Supplemental files are not converted into a PDF with the text figures and tables and will be downloaded as needed. Please note: Files that CANNOT be used include any TeX-type files, Bitmap (.bmp), PICT (.pict), photoshop (.psd), PowerPoint (.ppt), and CorelDRAW (.cdr). All files of figures made with these programs should be converted to GIF, TIFF, EPS, or JPEG. For additional information on submitting figures, see the Electronic Art Submission section below.
We hope you will find this new submission system helpful, and we look forward to receiving feedback so that we may continue to improve the system for manuscript submission and review. We are working to increase the types of files we can accept. Alternatively, manuscripts may continue to be submitted as hard copies (see the following instructions).
Hard Copy Submission. Manuscripts should be submitted to:
- Mark Hermodson, Editor
Protein Science
Department of Biochemistry
Purdue University
Biochemistry Building
West Lafayette IN 47907-1153, USA
Telephone: (765) 496-3459
Facsimile: (765) 496-3460
E-mail: proteinscience@purdue.edu Submit the original manuscript with TWO (2) sets of original, high-quality artwork plus four hard copies containing artwork suitable for reviewer evaluation (for a total of six (6) copies), and a diskette version containing the full manuscript (we cannot use TeX-type files). Where color figures are employed, high-quality copies with color illustrations must be provided for the reviewers. Each manuscript must be accompanied by a submission letter; three copies of any related paper by the authors that is either submitted or in press; and the area of expertise, full names, mailing addresses, telephone and fax numbers, and e-mail addresses of at least six qualified reviewers. Authors may disqualify up to two potential reviewers.
Review - A decision will be made within two weeks whether the paper is appropriate for review. Papers considered confirmatory or lacking sufficient originality will be returned without review. Most papers considered appropriate will be reviewed by at least two independent reviewers, one of whom may be a member of the Editorial Advisory Board. The Editor and/or the Associate Editors will make final selection of reviewers. If the reviewers disagree, or if in the judgment of the Editors the manuscript has not received adequate consideration, the manuscript and the reviewers?opinions may be submitted to a member of the Editorial Advisory Board for arbitration. Editorial decisions that result from this process are usually final. The inability to identify referees willing to review a manuscript may be considered grounds for rejection.
Revisions - When a manuscript requires revision, the author should return the manuscript to the Associate Editor or the Editor-in-Chief, as specified in the decision letter, with an accompanying letter indicating how he/she has responded to the reviewers?criticism and suggestions. Papers that have been returned for revisions should be returned within 30 days. Revisions returned after the requested period may be considered as new submissions. It is the policy of Protein Science that all new protein and nucleic acid sequences and atomic coordinates be deposited with the appropriate databases (see Sections 15 and 16 below).
Following these guidelines will facilitate peer review and technical preparation of your contribution. Manuscripts must be written in clear and concise English and must be submitted on 8 1/2 x 11" or A4 size high-quality paper, on one side of the sheet only, with double line spacing throughout (including title page, references, tables, and figure captions) and at least 1" (25 mm) margins on all sides. Standard "typewriter" fonts (e.g., Courier or Letter Gothic) that have uniform character spacing, not proportional spacing, are strongly preferred. Draft quality or illegible printing is unacceptable. Do not use word hyphenation or text justification options. Do not prepare the manuscript in a desktop-published form emulating printed journal style. An original and FOUR copies of the manuscript are required; high-quality photocopies are acceptable. Please observe the following order: Title page, Abstract (250 words or less), Introduction, Results, Discussion, Materials and methods, Acknowledgments, References, Tables, Figure legends.
Protein Science employs electronic publication methods in various ways, for both review and production purposes. If submitting by post, submission of manuscripts on diskette is required. Specify on the diskette label (a) filename and contents, (b) MS-DOS or Macintosh, and (c) the word processor used. Authors also must submit 5 paper (hard copy) manuscripts and printed figures when submitting a diskette at any stage if submitting by post.
After review and revision, the author must supply updated electronic files (via the online submission site). In the event revisions are returned by post, submit a diskette (3.5? zip, or CD) and at least 3 copies of the manuscript (including 2 copies of high-quality, camera-ready original artwork) incorporating all changes made during revision (hard copies of artwork are necessary even if submitting digital artwork). These will be submitted to the Publisher for production purposes. Supplementary material for publication in the Electronic Edition must be submitted electronically on a separate diskette (see Section 14 of Manuscript Elements) or on the online submission site (http://submit.proteinscience.org).
In addition to the printed edition, Protein Science is available as an online journal (http://www.proteinscience.org) which is scheduled to appear on the Internet even before the printed edition is mailed. Articles are posted in blocks as they are copy-edited. Tables of content and a list of forthcoming publications are posted well in advance in order to enable readers and subscribers to obtain a preview of the journal’s contents.
Manuscripts should be organized in the following way, although papers prepared otherwise will be considered if there are clear and compelling reasons for variation. All manuscript pages must be consecutively numbered.
1. Title page. The title should be concise and informative, but should avoid the use of acronyms and abbreviations. Include (a) full article title; (b) names and affiliations of all authors (matched by superscript numbers); (c) name, mailing address, telephone number, fax number, and e-mail address of the corresponding author; (d) running title of 50 characters or less; (e) list of total number of manuscript pages, supplementary material pages, tables, and figures; and (f) a note indicating that a diskette of the manuscript is included, and what environment and word processor are used; and (g) a description of supplementary material intended for publication in the Electronic Edition, including filenames.
2. Abstract and keywords. Include (a) an abstract of no more than 250 words and (b) four to eight keywords or short phrases for indexing that reflect the content and major thrust of the paper. The abstract should succinctly describe the objectives of the research, the experimental approach, and the major results and their significance. It must be self-explanatory and suitable for abstracting services such as Chemical Abstracts, Biosis, etc. Reference citations in the abstract should be avoided whenever possible and, if necessary, given in full. Avoid the use of abbreviations and acronyms in the abstract unless they are defined therein.
3. Abbreviations and symbols. Use standard abbreviations, acronyms, and symbols, and clearly define uncommon ones parenthetically within the text upon first appearance. The Journal will accept standard Journal of Biological Chemistry abbreviations. If abbreviations are particularly complex or numerous, they may be collected into an abbreviations footnote, which will appear on the first or second page of the printed article.
4. Introduction (no heading). The text of the paper begins on a new page. The Introduction should state the purpose of the investigation, the hypotheses tested, and the relationship to other work in the field. Avoid lengthy reviews of the literature.
5. Results. The results should be presented in a clear and concise manner, mentioning figures, kinemages, and tables that summarize or illustrate important findings.
6. Discussion. Briefly interpret the results and relate them to existing knowledge in the field, but do not merely restate the results or present reviews of the literature.
7. Materials and methods. Describe materials and methods briefly but in sufficient detail to allow others to repeat the experiments. Novel procedures should be described in detail, but published procedures should be referenced by a literature citation. If hazardous materials or dangerous procedures are employed, necessary precautions must be stated.
8. Electronic supplementary material. Describe briefly the supplementary material submitted on the diskette with the manuscript that is intended to be available over the Internet as part of the Electronic Edition of Protein Science. The names of all files should be given so that World Wide Web hyperlinks to them can be established within the Electronic Edition. All files provided must conform to the instructions in Sections 14 and 15 below.
9. Acknowledgments. Research grants or other funding (including grant or project numbers as appropriate) may be acknowledged, as may significant contributions of individuals providing scholarly, technical, or clerical assistance.
10. References. Authors are asked to avoid extensive references. Protein Science uses the author-date reference style, with in-text citations within parentheses (Hecth et al. 1990) and an unnumbered reference list alphabetized by first author surname. Surnames and initials of all authors and editors of cited works must be provided. All references cited in the text must be included in the References section and vice versa. Titles of journal articles and book chapters must be included; journal titles are abbreviated according to Chemical Abstracts Service Source Index, 1985. Page numbers must be inclusive. If a pre-existing coordinate set is used either in the research or to produce a figure, its databank file code should be given either in the caption or the Materials and methods section, in addition to whatever literature references may be appropriate.
Please prepare the References list according to the following examples. Note especially:
(a) the punctuation of authors (periods are used after initials, comma used between surname and initials and to separate authors);
(b) publication dates not enclosed in parentheses;
(c) titles required for all journal articles and individual book chapters cited;
(d) only initial word and proper nouns capitalized in article, chapter, and book titles;
(e) periods are used after abbreviated journal titles; and
(f) full page ranges are required (not simply beginning page of article).
EXAMPLES:
Journal Articles
Hecht, M.H., Richardson J.S., Richardson D.C., and Ogden R.C. 1990. De novo design, expression, and characterization of Felix: A four-helix bundle protein of native-like sequence. Science 249: 884-891.
Cierpicki, T., Bania, J., and Otlewski, J. 2000. NMR solution structure of Apis mellifera chymotrypsin/cathepsin G inhibitor-1 (AMCI-1): Structural similarity with Ascaris protease inhibitors. Protein Sci. 9: 976-984.
Books and Book Chapters
Fischmann, D.A., Vaughn, K., Weber, F., and Einheber, S. 1991. Myosis binding proteins: Intracellular members of the immunoglobulin super family. In Frontiers of muscle research: Muscle contraction and muscle dystrophy, 2d ed. (eds. E. Ozawa et al.), pp. 211-222. Elsevier, Amsterdam.
Perutz, M. 1990. Mechanisms of cooperativity and allosteric regulation in proteins. Cambridge University Press, Cambridge, UK.
Sambrook, J., Fritsch, E.F., and Maniatis, T. 1989. Molecular cloning: A laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
Annual Review of Biochemistry, Advances in Protein Chemistry, Methods in Enzymology, and similar works should be cited in serial publications format. Unpublished data and personal communications must be cited as such parenthetically in the text and must not appear in the References section. All citations of personal communications must be certified by letter from the communicating author.
11. Tables. All tables must be cited in the text in numerical order and the approximate position of each indicated in the margin. Each table should be typed double-spaced on a separate page (or, if exceptionally large or requiring special symbols or unusual treatment, the table should be submitted camera-ready). Tables should be numbered consecutively with Arabic numerals and grouped together after the References section. A short explanatory title, column headings, and (if necessary) footnotes should make the table intelligible without reference to the text. Vertical and diagonal rules should not be used in tables; instead, indentation and vertical or horizontal space should be used to group data. Large tables of an appendix nature may be considered for electronic publication in the Electronic Edition. If tables are reprinted from another source or if data included are from another source, permission to reprint is required.
12. Figure legends. Type all figure legends double-spaced. Figure legends should be brief and should not contain methods. Each figure legend must begin with a short statement of the general content of the figure. Symbols indicated in the figure must be identified in the legend text. If figures are reprinted from another source, permission to reprint is required. Explain all symbols and abbreviations.
13. Figures (artwork). Please also read the Electronic Art Submission section. Manuscripts submitted by post must include six sets of figures as high-quality glossy prints. We also request that authors provide electronic copies of the figures with the manuscript (in .TIFF, .JPEG, .GIF, or .EPS format); this enables the staff to more quickly enter the manuscript into our online system and will expedite the conversion process. Electronic figures and hard copies of figures should be the exact same size and note that only .TIFF and .EPS files can be used for print publication purposes (see the Electronic Art Submission section). Hard copy submissions lacking electronic figures will have their figures scanned. For the best reproduction, halftones should be high contrast. Line drawings, graphs, charts, and chemical formulae should be professionally prepared and labeled. Indicate magnification with a bar scale. Multipart figures should be submitted as one composite. Authors submitting color figures as essential data for review with manuscripts undertake to pay the publication costs of four-color artwork. Price estimates are supplied upon acceptance of the paper.
Label the back of each figure with the first author's name, figure number, and an indication of "top." (Electronic copies should be labeled with the first authors name and the figure number, for example Smith_Fig1). Number figures consecutively in the order in which they are referred to in the text. The figures sizes will be adjusted to fit the journal format; therefore, please keep labels, symbols, and other callout devices in proportion to the figure size and detail.
Figures should be kept to the minimum necessary to document results or methods that cannot be described in the text. Linear plots, particularly reciprocal velocity plots, should be avoided and replaced in the text in terms of slopes, intercepts, and standard deviations. All figures must be cited in the text in numerical order and the approximate position of each indicated in the margin. All figures must be camera-ready and have a professional appearance. Most figures will be published in a single column of the Journal; use lettering of sufficient size to be legible after reduction (i.e., labels should be no smaller than 1.5? mm high in the reduced figure). Lettering of all figures within the article should be of uniform style (preferably a sans serif typeface; typewritten labels are strongly discouraged), and should not easily flake off. Use uppercase letters A, B, C, etc. to identify individual parts of multipart figures. Poor-quality figures or illegible labeling may result in delayed acceptance and publication.
Check figures carefully before submission to be sure that proper versions are being sent and that there are no labeling errors. Each figure must be clearly identified (on the reverse) by author, article title, (Arabic) figure number, and an indication of the top of the figure. The figures should ordinarily not exceed 8??11" or A4 paper size and must be enclosed in a protective envelope. Submit all original illustrations in duplicate.
Stereo pairs must be mounted side by side. Corresponding points of the two images should be 6.3 cm apart.
The use of color is encouraged and justified when it significantly enhances communication and the understanding of the scientific data reported in the original manuscript. Protein Science provides authors with a significant subsidy for the reproduction of color figures, but authors are asked to contribute $350 per color page (or plate), which is considerably less than actual production costs. Note: Authors can save considerable costs to themselves and the journal by photographing Figure 1A, B and C on a single page (or plate) rather than providing these on three separate pages (or plates). For instance, if one figure has three panels and each panel is on a separate page (or plate), then this figure will be counted as three color costs ($350 x 3). To avoid this, provide all three panels on one page (or plate). For best reproduction, bright, clear colors should be used; muted, "muddy" colors produce less satisfactory results. Dark colors against a dark background do not reproduce well. Lemon yellow (especially against a white background) tends to disappear; use slightly greenish or golden yellows instead.
Kinemages of selected appropriate subjects will be published electronically at the Editor’s discretion. For information, see Section 14.
**Our printer can only accept electronic art as TIFF or EPS files. We also require two hardcopies of each figure at final acceptance to properly size and color match the electronic figures. Detailed instructions which may aid you in converting files to those suitable for processing by our printer are provided below. **
Please note we CANNOT accept Canvas, PowerPoint, SuperPaint, Corel Draw, Harvard Graphics, or Excel graphics because they are not compatible with our printer's output devices.
If you have created your artwork electronically, please send the electronic files saved according to the detailed instructions below.
- For final publication purposes, we can only accept electronic art as an EPS or TIFF file. See specific instructions below on saving your files in the most common programs. (For reviewing purposes, other file types are acceptable, as outlined above under Manuscript Submission and Review.)
- UPON FINAL ACCEPTANCE, AUTHORS MUST PROVIDE TWO (2) HIGH-QUALITY, CAMERA-READY COPY PRINTS WITH THEIR ELECTRONIC FILES for cases when electronic versions of the art fail and as an additional check for the quality of the electronic version for publication. Label the figures on the back with the manuscript number, first author's name and figure number. Always include a printout of the art at the same size it is saved on the disk. Do not enlarge or reduce the printout. This is important in case the electronic file can not be read and to make sure that the file on the disk is the same as the print provided. This printout is also used to size the artwork. If the art is in color, please provide a high quality color print so the print may be used as a guide to match the colors.
- Please make sure that your files are properly labeled by first author's name/figure number (for example, Smith_Fig1, Smith_Fig5) and that printouts are properly labeled and correspond to the file name. Also indicate the programs used to generate the artwork.
- Send only final electronic art files. DO NOT EMBED FIGURES WITHIN THE MANUSCRIPT TEXT FILES.
- Use Helvetica typeface in point sizes no smaller than 8 pt and no larger than 10 pt, except for the main callouts (e.g. A, B, C), which should be 12 pt.
Generating Electronic Artwork from Original Glossy Prints:(For artwork that was not created electronically for which you wish to provide an electronic version)
All photographs need to be scanned at a resolution of at least 350 dpi. Photographs should be saved as a TIFF file (not EPS) in Photoshop. If the figure is in color, make sure it is converted to CMYK (not RGB or Pantone) and follow specifications below for color art. If the artwork that contains the photograph also contains line art (even one or two text characters), you need to follow the specifications below for combo art. When in doubt with halftone art, please follow the specs for combo art.
All line art figures need to be scanned at a resolution of at least 1200 dpi in bitmap mode. Line art should be saved as EPS files. If the line art is in color, make sure it is converted to CMYK (not RGB or Pantone) and follow specifications below for color art.
All color art should be scanned or produced at 350 dpi in CMYK mode and saved as TIFF files. Always save your color scans in the CMYK color space. Never submit color electronic files in RGB mode.
Please send us the original electronic files for any artwork that has been created using these programs. In order to use these files, please save the files using the specific instructions below.
Once again, please note we CANNOT accept Canvas, PowerPoint, SuperPaint, Corel Draw, Harvard Graphics, or Excel graphics because they are not compatible with our printer's output devices.
Adobe Illustrator -
- Under the File menu, choose save as, format (Illustrator EPS)
- Click save and a window will come up
- Click compatibility 6.0
- Under options, click include document fonts and click include document thumbnails
- Preview should be checked as 8-bit Macintosh
- Click OK
- In case we have trouble with your file, please download all fonts that were used in creating your artwork onto the disk.
The preview box is very important. Make sure it is checked off as 8-bit Macintosh.
If the artwork is in color, please make sure to create the artwork using CMYK colors.
If TIFF images are transferred into Illustrator, please make sure to supply the TIFF images separately on the disk as well. Create a folder on the disk labeled (Illustrator TIFFs). When saving the file, make sure "include placed files" is checked in the options box.
Adobe Photoshop -
- Under the File menu, choose save as, format (TIFF)
- Click save
- Under TIFF options check Macintosh, do not check LZW compression
- Click OK
Do not save your files as EPS files.
If the artwork is in color, please make sure to create the artwork using CMYK colors (not RGB or Pantone colors). Please make sure the resolution is at 350 dpi.
If you have any questions on how to save your artwork, please contact the US office at proteinscience@purdue.edu .
A Word About Electronic Art Submission:
Cold Spring Harbor Laboratory Press makes every effort to use the electronic files from the authors. We are well equipped and trained with many of the graphics software programs that are used in the scientific community. Adobe Illustrator 6.0 or newer and Adobe Photoshop 4.0 or newer are the main "true" graphics programs that all publishers and printing industries support. The file formats generated by these programs are universal and can easily be read by high-end imagesetters. Canvas, Corel Draw, Powerpoint, Harvard Graphics, and SuperPaint are typical graphics programs that excel at creating visual presentations, such as slides, and overheads. However, they do not always export in file formats that can be printed through high-end imagesetters.
Printers print in CMYK. Most authors submit their files in RGB. When these files are converted to printing industry standards, the colors often change completely. This is why it is very important for the artwork to be created as CMYK and for a hard copy of the artwork to be submitted for comparison. In the event that we cannot use the electronic file, a film from the hard copy image will be created. Also, note that our printer will not accept a digital art file unless it is accompanied by high-quality reflective art that matches exactly, as well.
It should be stressed again that electronic figures must be submitted using the following programs: Illustrator and Photoshop. We hope the other graphics programs will eventually support printing industry standards as well.
14. Supplementary material to appear in the Electronic Edition. An author may request that lengthy tables and other data appear on the Protein Science Electronic Edition; such material will not be printed in the Journal. In such cases, authors should include a short paragraph after the Materials and methods section entitled “Electronic Supplementary Material?with a brief description of the content. Supply supplementary material in electronic form (standard MS-DOS or Macintosh diskettes) with the manuscript and describe it briefly (including filenames) at the bottom of the article title page. Electronic publication of supplementary material is strictly at the Editor’s discretion.
Supplementary tables for the Electronic Edition should be constructed, if possible, in a manner that will permit them to be read by common computer programs. They should be in plain ASCII without word processor formatting information.The tables should be constructed using a nonproportional spacing font such as Courier with close attention to spacing. Each diskette file should include a header paragraph specifying page orientation, typeface, and font size used in viewing the table.
If it is not practical to construct supplementary tables in the ASCII format because special characters or elaborate layouts are essential for the presentation of the data, then formatted tables must be provided that can be read by standard word processor or spreadsheet software using IBM or Macintosh compatible computers.
Data that are best viewed in a graphical form should be submitted suitable for viewing by means of standard spreadsheet software. The files should be in plain ASCII text arranged as columns separated by tabs or commas. Each column should be identified by a short name or letter set off by double quotation marks, e.g. "X", "Y1", "Y2", etc. There should be a header paragraph with each line (80 characters maximum) enclosed in quotation marks that gives the titles of the X and Y axes and any other information that may be useful to the reader. Any other means of electronic publication of graphical material should be cleared in advance by the Editor and the Electronic Publishing Coordinator.
Graphical data that cannot be readily prepared as ASCII text may be provided as bitmap images, Microcal Origin data files, Jandel SigmaPlot data files, Lotus 123 or Excel spreadsheet files, or as standard word processing files containing embedded images. Any other means of electronic publication of graphical materials must be cleared in advance by the Editor and the Electronic Publishing Coordinator.
Authors of papers that describe newly determined or modeled three-dimensional structures are encouraged to provide coordinate files if they will not be available in the Brookhaven Protein Data Bank (PDB) at the time of publication. Coordinate files should conform to the Brookhaven PDB format. All files names should be in the DOS 8.3 filename format with the extension *.xyz. If the PDB 4-character identifier is known, it should be included in the filename prefix. A description of the structure, its method of determination, and other pertinent information should be included as remarks at the beginning of each file.
Authors are also encouraged to submit computer programs and software that may be of value to protein scientists. The programs may be included as ASCII source-code files or as executable binary files. In either case, an ASCII text file that describes vital information, such as compiler, operating system, computer platform, and instructions for compiling/executing, should accompany the programs.
Kinemages, illustrations using interactive graphics, may also be used to enhance articles. Kinemages, and an early version of the MAGE display program, are described in “The kinemage: A tool for scientific communication,?by D.C. Richardson and J.S. Richardson (1992, Protein Sci. 1: 3-9). Authors should create their own kinemages, using the most up-to-date versions of the PREKIN and MAGE programs and the instructions available from the Protein Science Web site, and submit them along with the manuscript for peer review. The kinemages should be in a plain text file with the suffix ".kin", and its presence should be noted in the cover letter.
Authors should submit their kinemages and other material meant primarily for machine readability in electronic form. In the letter of submission, include a brief description of all Electronic Edition materials and indicate their desired treatment (provide this information as well at the bottom of the title page of the manuscript, including filenames). Electronic publication of such material is subject to availability of space and to the Editor’s discretion.
While abstract and reference files for the Electronic Edition will be prepared by the Journal, preparation and submission of material for publication only in the Electronic Edition are the responsibility of the author. Such material includes coordinate lists and program source code and will normally appear only in the Electronic Edition, not in the printed Journal.
15. Data Deposition Requirements. Protein and Nucleic Acid Coordinates. Atomic coordinates for new three-dimensional protein structures must be deposited, before acceptance of the manuscript, with the Brookhaven Protein Data Bank (by FTP at ftp.rcsb.org or WWW at www.rcsb.org; fill out /pub/dep_form.txt) in order for a file identification code to be assigned and published in the article. Protein Science requires, as a condition for publication, that coordinates be publicly released no later than the publication date of the article. Coordinates also need to be made available for the reviewers; therefore, they should be submitted along with the original manuscript, on diskette. They will be treated confidentially, as any information in the papers under review.
Authors are strongly encouraged, but not required, to deposit in the Protein Data Bank X-ray structure factors, NMR assignments and lists of NOE constraints and related data.
16. Data deposition requirements. Protein and Nucleic Acid Sequences. Protein sequence data must be deposited, prior to manuscript submission, with the Protein Identification Resource (PIR) (National Biomedical Research Foundation, Georgetown University Medical Center, Washington, DC 20007, USA). Authors must certify in writing that this sequence has been scanned against this (or other comparable) database(s) and that all identities or significant similarities, including species variation, with other entries have been reported in the manuscript.
Nucleotide sequence data must be included as part of manuscript submission and also, prior to manuscript acceptance, must be sent by the author to GenBank Submissions (M.S. K-710, Los Alamos National Laboratory, Los Alamos, NM 87545, USA).
| Checklist for Manuscript Submission |
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Full article title, authors, affiliations, and corresponding author’s mailing address should be on a separate page. |
| ___ |
Eliminate nonstandard abbreviations in titles. |
| ___ |
Supply full author names. |
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Indicate institutional affiliation for all authors. |
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Provide complete mailing address for all affiliations. |
| ___ |
Identify corresponding author and supply telephone number, fax number, and E-mail address. |
| ___ |
Indicate on title page how the text was saved on your computer. Example: Word Perfect 5.1 for PC. |
| ___ |
Abstract and keywords should be on a separate page. |
| ___ |
Do not cite references, tables, or figures in the abstract. |
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Include written permission for any borrowed or modified figures or tables from the copyright holder. |
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Supply full manufacturer names and addresses (city and state or country) for all brand name products cited in text. |
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Make sure English is grammatically correct and idiomatic. |
| ___ |
Cite tables and figures in the text in Arabic numeral order. |
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Check that references are in the style of the journal, accurate, and cited in the text. |
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Do not cite materials submitted but not yet accepted for publication as references. Instead, cite in text as unpublished work. |
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Supply tables on separate pages. |
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Tables should not be saved as cells or in a graphic program. | |
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List figure legends as a group on a separate page. |
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Make sure figures are of good quality, either camera-ready or on glossy-photo type paper. |
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Label each figure on the back with the first author’s last name, and figure number. Please indicate the top of the figure. |
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Save text, tables, figure legends, etc. on one disk if possible. |
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| Electronic Supplementary Material |
| ___ |
Does the cover letter indicate the existence of and a description of electronic supplementary material? |
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Is the supplementary material indicated on the title page of the article? |
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Is there a section after the Materials and methods section entitled Electronic supplementary material (ESM)? |
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Does the ESM section briefly and adequately describe the supplementary material? |
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Does the ESM section include a complete list of file names supplied on the disk? |
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Are all of the supplementary files on a separate disk? |
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Are the names of these files in the DOS "8.3" format? |
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Do the names of kinemage files conform to the specified Naming Conventions (see also: www.faseb.org/protein/ kinemages/KinemageNamingConventions. html)? |
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Are the ESM files saved in ASCII format? If not, indicate the software (version) used to create each file. |
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Do the ESM files satisfy the requirements as set down in Section 14 of Manuscript Elements and Order in the Instructions to Authors? | |
Editorial Board
Edited By Mark Hermodson,
Editor-in-Chief, Purdue University;
Stephen H. Bryant, NIH;
Brian W. Matthews, University of Oregon;
arol V. Robinson, Cambridge University
Hard Copy Submission. Manuscripts should be submitted to:
- Mark Hermodson, Editor
Protein Science
Department of Biochemistry
Purdue University
Biochemistry Building
West Lafayette IN 47907-1153, USA
Telephone: (765) 496-3459
Facsimile: (765) 496-3460
E-mail: proteinscience@purdue.edu
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