期刊名称:ELECTROPHORESIS
期刊简介(About the journal)
投稿须知(Instructions to Authors)
编辑部信息(Editorial Board)
About the journal
ELECTROPHORESIS is the world's leading journal for new analytical and preparative methods and for innovative applications on all aspects of electrophoresis. In life sciences, electrophoresis is perhaps the most ingenious and important method with ubiquitous applications in both research and routine. ELECTROPHORESIS will continue to serve as indispensable vehicle for the dissemination of crucial advances by covering all operative approaches from gels through capillaries to chips. Original papers or short communications are welcome from all areas of life sciences and biomedicine. Review articles are normally invited by the Editors. Authors wishing to submit a review should send a brief outline of its contents to one of the Editors.
Instructions to Authors
Authors are requested to follow these instructions carefully. Manuscripts not prepared accordingly will not be accepted. General Electrophoresis is an international journal that publishes original manuscripts on all aspects of electrophoresis. Topics include new or improved analytical and preparative methods, development of theory, and innovative applications of electrophoretic methods in the study of nucleic acids, proteins, and other compounds. Contributions are welcome from the fields of biochemistry, molecular and cell biology, genetics, immunology, microbiology, clinical chemistry, forensics, food science and other disciplines. Electrophoresis publishes articles in English. Manuscripts must be grammatically and linguistically correct, and authors less familiar with English usage are advised to seek the help of English-speaking colleagues. American spelling is preferred. Types of manuscripts Three types of manuscripts are accepted for publication: (i) Original papers describing complete investigations. (ii) Short communications describing results that are brief, timely and/or of such importance that rapid release is warranted. These manuscripts should bear the words Short communication immediately above the title on the first page. They should not be subdivided into titled sections but should be written in a continuous style. (iii) Review articles will normally be invited by the Editors. Authors wishing to submit a review article should send a brief outline of its contents in duplicate to one of the Editors before the manuscript is drafted. Submission of manuscripts Required are: the original manuscript (with original figures and tables) plus three complete copies of good quality. The author vouches that the work has not been published elsewhere, either completely, in part, or in any other form and that the manuscript has not been submitted to another journal. The submitting author (listed under Correspondence) accepts the responsibility of having included as coauthors all persons appropriate and none inappropriate. The submitting author certifies that all coauthors have seen a draft copy of the manuscript and agree with its publication. Manuscript should be submitted to editors whose area of expertise is closest to the topic of the paper. On publication the papers will be allocated to one of the five subject categories: (i) general, (ii) nucleic acids, (iii) capillary electrophoresis and capillary electrochromatography (CE and CEC), (iv) miniaturization, (v) proteomics and two-dimensional electrophoresis (2-DE). In ambiguous cases authors may indicate the preferred subject category for publication. Professor Yoshinobu Baba Department of Medical Chemistry The University of Tokushima, Shomachi Tokushima 770-8505 (Japan) E-mail: ymttbaba@ph.tokushima-u.ac.jp Professor Petr Boc¡¦ ek Institute of Analytical Chemistry Czech Academy of Sciences, Vever˘¨ª 97 CZ-61142 Brno (Czech Republic) E-mail: bocek@iach.cz Fax: +420-5-4121-2113 Dr. Andreas Chrambach Bldg. 10, Rm. 9D50, National Institutes of Health Bethesda, MD 20892-1580 (USA) E-mail: acc@cu.nih.gov Fax: +1-301-402-0263 Professor Ziad El Rassi Department of Chemistry, Oklahoma State University Stillwater, OK 74078-3071 (USA) E-mail: zelrassi@biochem.okstate.edu Fax: +1-405-744-6007 Professor Jörg T. Epplen Molecular Human Genetics Ruhr-Universität Bochum, Geb. MA 5/142 D-44780 Bochum (Germany) E-mail: joerg.t.epplen@ruhr-uni-bochum.de Fax: +49-234-32-14196 Dr. Frantisek Foret Barnett Institute, 341 Mugar Bldg., 360 Huntington Ave., Boston, MA 02115 (USA) E-mail: fforet@lynx.dac.neu.edu Professor James P. Landers Department of Chemistry, McCormick Road, University of Virginia, Charlottesville, VA 22901 (USA) E-mail: jpl5l@virginia.edu Professor Takashi Manabe Department of Chemistry, Faculty of Science Ehime University Matsuyama-City, 790-8577, Japan E-mail: manabet@dpc.ehime-u.ac.jp Fax: +81-89-927-9590 Professor Bertold J. Radola Institute of Food Technology and Analytical Chemistry Technical University Munich, Weihenstephaner Steig 23 D-85350 Freising (Germany) E-mail: radola@wzw.tum.de Fax: +49-8161-12962 Professor Nancy C. Stellwagen Department of Biochemistry, The University of Iowa 4403 Bowen Sci. Bldg. Iowa City, IA 52242-1109 (USA) E-mail: stellwag@blue.weeg.uiowa.edu Fax: +1-319-335-9570 Refereeing All contributions are evaluated by referees whose names are not normally disclosed to authors. Authors may suggest up to five potential referees (including complete addresses and fax numbers) as well as individuals whom they wish to be excluded from the review process. On acceptance, papers may be subjected to editorial changes. A revised paper will retain its original date of receipt only if it is resubmitted to the Editors within two months after revision was requested. Responsibility for the factual accuracy of a paper rests entirely with the author. Arrangement of manuscripts Manuscripts must be typewritten with double spacing (including footnotes, references, tables, legends, etc.) on paper not exceeding 30 cm in height (Standard A4 or 8.511 inch paper is appropriate) leaving margins of 3.5 cm on the right and left sides. Contents of first page of manuscript The first page of the manuscript should contain only the following: 1) Title of the paper containing only the keywords pertaining to the subject matter. No abbreviations should be used in the title. 2) Full names (including first name) of the authors and the name of the institute. If the publication originates from several institutes the affiliations of all authors should be clearly stated by using superscript numbers after the name and before the institute. 3) A shortened version of the title not exceeding 70 letters (running title). 4) Name and full postal address of the author to whom all correspondence (including galley proofs) is to be sent. E-mail code and fax number should be included to speed up communication. 5) A list of abbreviations used in the paper (for correct abbreviations in biochemistry, please consult the IUB compendium Biochemical Nomenclature and Related Documents, 1978, The Biochemical Society, London WC1R 5DP, GB), excluding standard abbreviations (see list of Standard Abbreviations and paragraph Abbreviations below). 6) Keywords (maximally 5), which will be used for compiling the subject index. Summary The second and (if necessary) third page of the manuscript should contain the summary only. This must be self-explanatory and intelligible without reference to the text. Normally, it should not exceed 200 words. Division into sections Manuscripts should be divided into the following sections: 1 Introduction: containing a description of the problem under investigation and a brief survey of the existing literature on the subject. 2 Materials and methods: for special materials and equipment, manufacturer¡¯s name and location should be provided. 3 Results 4 Discussion 5 References Sections 3 and 4 may be combined and should then be followed by a short section entitled Concluding remarks. Subdivisions of sections should be indicated by subheadings. References References should be numbered sequentially in the order in which they are cited in the text. The numbers should be set in brackets, thus [2, 13]. References are to be collected in numerical order at the end of the manuscript under the heading References; they should also be typed with double spacing throughout. Titles of journals should be abbreviated according to the practice of Chemical Abstracts. The abbreviated title and the volume number should be in italics. Please note the following examples. Journals: [1] Lidsky, M. D., Sharp, J. T., Rudel, M. L., Arch. Biochem. Biophys. 1967, 121, 491¨C502. Other serial publications such as Advances in Protein Chemistry should be cited in the same manner as journals. Books: [2] Elves, M. W., The Lymphocytes, Lloyd-Luke Ltd., London 1972, pp. 274¨C289. Chapter in a book: [3] Möller, E., Greaves, M. F., in: Mäkelä, O., Cross, A., Kosunen, T. U. (Eds.), Cell Interactions and Receptor Antibodies in Immune Responses, Academic Press, New York 1971, pp. 101¨C125. Allusions to unpublished observations, papers to be published or submitted for publication and the like should be part of the text, either in parentheses or as footnotes. Material in press should be entered under references. Responsibility for the accuracy of bibliographic references rests entirely with the author. Footnotes Footnotes, i.e., explanations or comments on the text, should be indicated by an asterisk* and written at the bottom of the page on which the asterisk appears in the text. Acknowledgement Acknowledgements as well as information regarding funding sources should be provided on a separate page and will appear at the end of the text (before References). Tables Tables with suitable captions at the top and numbered with Arabic numerals should be collected at the end of the text on separate sheets (one page per Table). Column headings should be kept as brief as possible and indicate units. Footnotes to tables should be indicateda), b), c) etc. and typed on the same page as the table. Figures and legends Diagrams and photographs should also be submitted on separate pages at the end of the article (new page for each figure). Three copies of each figure are required. Figures should be numbered consecutively with Arabic numerals in the order of their appearance. Photographs should be fine quality, large glossy prints suitable for reproduction. Figures should not be larger than the manuscript paper. Numbers and symbols inscribed must be large enough to be legible after reduction in size. In electropherograms presented horizontally, the anode should be at the left while in vertical presentations the anode should be at the bottom. Two-dimensional presentations, e.g. with isoelectric focusing and sodium dodecyl sulfate ¨C electrophoresis in the two dimensions, are thus presented consistent with the standard coordinate system. Each figure is to be accompanied by a legend, which should be self-explanatory. The legends should not appear under the figures but be collected and typewritten with double spacing on a separate page. Structural formulas Structural formulas should be drawn in the manuscript at the position where they belong. They may be numbered in the order of their appearance with Arabic numerals in parentheses. Equations Mathematical and chemical equations are to be written in the manuscript at the place in which they belong and should be marked by Arabic numerals in parentheses in the right margin in the order of their appearance. Abbreviations Abbreviations are hindrances to a reader working in a field other than that of the author, and to abstractors. Therefore, their use should be restricted to a minimum. Abbreviations should be introduced only when repeated use is forthcoming. Abbreviations used only in a table or a figure may be defined in the legend. No abbreviations should be used in the title and keywords. If standard abbreviations are used in the Summary they must be defined when first introduced. If nonstandard abbreviations are used in the Summary they should be defined in the Summary, in the list of abbreviations of the manuscript, as well as upon their first use in the body of the paper. A list of standard abbreviations which may be used in the text without definition is included with these Instructions. Electronic manuscripts Please follow the Instructions to authors when preparing the manuscript on disk and ensure that data are given in the order and the correct style for the journal as outlined above. Data should be typed unjustified, without hyphenation except for compound words. Use carriage returns (¿) only to end headings and paragraphs; spacing will by introduced by the typesetter. Do not use the space bar to make indents; where these are required (e.g. tables) use the TAB key. If working in Word for Windows, please create special characters through Insert/Symbol. Figures supplied electronically should have the following resolution: Figures should be in TIFF format. Type Resolution Graph 800¨C1200 DPI Photos 800¨C 400 DPI Color (only CMYK) 300¨C 400 DPI Figure legends and tables (in this order) should be given in a separate file. Please check the final version of your manuscript carefully to avoid errors in the typeset copy. The disk must be accompanied by a printout of the final version (see section 3 ¡°Submission of Manuscripts¡± for the number of copies required). If the disk and hard copy do not match, the hard copy will be used for further processing. Please submit your text on disks formatted for DOS: With Macintosh systems, files must be saved as Mac for DOS. The data should be copied onto a newly formatted disk. To avoid confusion only the final updated version should be sent. Data files only should be given; for technical reasons disks containing programs will not be processed. The disk itself should be clearly marked with: the name of the correspondence author, the date, the word processing program and its version number and, when available, the manuscript number. Do not include any copyrighted material (e.g. software) on the disk as this can create difficulties with customs clearance. Please ensure that the disk is properly packed to avoid damage in transit. Disks will not be returned; therefore, a copy should be kept for subsequent questions or revisions. Proofs and reprints Authors will receive two sets of proofs before publication. One set should be returned promptly by fax and the other by express air mail. Authors will be charged for extensive alterations. Reprints can be ordered at prices shown on the reprint order form which will be sent with the proofs. Upon publication the submitting author (listed under Correspondence) will receive a complimentary copy of the issue containing the article. Standard abbreviations The abbreviations listed below may be used without definition in the articles published in Electrophoresis. No abbreviations should be used in the title and keywords. If standard abbreviations are used in the Summary they must be defined when first introduced. If other than standard abbreviations are used in the Summary they should be defined in the Summary, in the list of abbreviations of the manuscript, as well as upon their first use in the body of the paper. A absorbance ACES 2-[(2-amino-2-oxoethyl)amino] ethanesulfonic acid ACN acetonitrile A/D analog to digital converter API atmospheric pressure ionization BGE background electrolyte Bis N,N¡¯-methylenebisacrylamide bp base pairs BSA bovine serum albumin %C cross-linking agent (g/100 mL) / %T CAPS 3-(cyclohexylamino)-1-propanesulfonic acid CBB Coomassie Brilliant Blue CCD charge-coupled device CD cyclodextrin CE capillary electrophoresis CEC capillary electrochromatography CHAPS 3-[(3-cholamidopropyl)dimethylammonio]- 1-propanesulfonate CHEF contour-clamped homogeneous electric field CHES 2-(N-cyclohexylamino)ethanesulfonic acid CID collision-induced dissociation CIEF capillary isoelectric focusing CMC critical micelle concentration Con A concanavalin A cpm counts per minute CTAB cetyltrimethylammonium bromide CV coefficient of variation CZE capillary zone electrophoresis 1-D one-dimensional 2-D two-dimensional Da dalton (molecular mass) 2-DE two-dimensional electrophoresis DGGE denaturing gradient gel electrophoresis DMEM Dulbecco¡¯s modified Eagle medium DMF N,N-dimethylformamide DMSO dimethyl sulfoxide DOC sodium deoxycholate DP degree of polymerization dsDNA double-stranded DNA DTT dithiothreitol ECL enhanced chemiluminescence EDTA ethylenediaminetetraacetic acid EEO electroendosmosis EGTA ethylene glycol-bis(ß-aminoethyl ether)-N,N,N¡¯,N¡¯-tetraacetic acid EKC electrokinetic chromatography ELISA enzyme-linked immunosorbent assay EOF electroosmotic flow ESI electrospray ionization EST expressed sequence tag FAB fast atomic bombardment FIGE field inversion gel electrophoresis FITC fluorescein isothiocyanate GC gas chromatography HEPES N-(2-hydroxyethyl)piperazine- 2¡¯-(2-ethanesulfonic acid) HPCE high-performance capillary electrophoresis HPLC high-performance liquid chromatography HSA human serum albumin HVR hypervariable region ICAT isotope coded affinity tag ID inside diameter IEF isoelectric focusing IPG immobilized pH gradient IT ion trap ITP isotachophoresis kbp kilobase pairs kDa kilodalton (molecular mass) LC liquid chromatography LIF laser-induced fluorescence LOD limit of detection LOQ limit of quantitation mAb monoclonal antibody MALDIMS matrix assisted laser desorption/ionization ¨C mass spectrometry Mbp megabase pairs MCS multiple cloning site MEKC micellar electrokinetic capillary chromatography MES 2-(N-morpholino) ethanesulfonic acid MOPS 3-(N-morpholino) propanesulfonic acid Mr relative molecular mass (dimensionless) MS mass spectrometry MS/MS tandem mass spectrometry NC nitrocellulose NEPHGE nonequilibrium pH gradient electrophoresis NMR nuclear magnetic resonance NP-40 Nonidet P-40 OD outside diameter OFAGE orthogonal field alternation gel electrophoresis ORF open reading frame PAGE polyacrylamide gel electrophoresis PBS phosphate-buffered saline PC personal computer PCR polymerase chain reaction PEG polyethylene glycol PFGE pulsed field gel electrophoresis PFU plaque-forming units pI isoelectric point PMS phenazine methosulfate PMSF phenylmethylsulfonyl fluoride PSD post-source decay PTFE polytetrafluoroethylene PTH phenylthiohydantoin PVDF polyvinylidene difluoride PVP polyvinylpyrrolidone RAPD randomly amplified polymorphic DNA RFLP restriction fragment length polymorphism RIA radioimmunoassay RP reversed phase rpm revolutions per minute RPMI Roswell Park Memorial Institute RSD relative standard deviation SD standard deviation SDS sodium dodecyl sulfate SEM standard error of the mean SIM selected ion monitoring S/N signal-to-noise ratio SPE solid-phase extraction SSCP single-strand conformation polymorphism ssDNA single-stranded DNA SSP sample spot number STR short tandem repeat %T total gel concentration (acrylamide plus cross-linking agent; g/100 mL) TBS Tris-buffered saline TCA trichloroacetic acid TEMED N,N,N¡¯N¡¯-tetramethylethylenediamine TFA trifluoroacetic acid TGGE temperature gradient gel electrophoresis THF tetrahydrofuran TLC thin-layer chromatography TOF time of flight Tris tris(hydroxymethyl)aminomethane U unit UV ultraviolet Vh volt hours VNTR variable number of tandem repeats WWW World Wide Web YAC yeast artifical chromosome
Editorial Board
Editor-in-Chief Bertold J. Radola (Freising, Germany) Senior Deputy Editors Petr Boc¡¦ ek (Brno, Czech Republic) Andreas Chrambach (Bethesda, MD, USA) Ziad El Rassi (Stillwater, OK, USA) Deputy Editors Yoshinobu Baba (Tokushima, Japan) Jörg T. Epplen (Bochum, Germany) Frantisek Foret (Brno, Czech Republic) James P. Landers (Charlottesville, VA, USA) Takashi Manabe (Matsuyama, Japan) Nancy C. Stellwagen (Iowa City, IA, USA) Editorial Board D. W. Armstrong (Ames) A. E. Barron (Evanston) J. L. Beckers (Eindhoven) P. Cash (Aberdeen) B. Chankvetadze (M¨¹nster) L. A. Colon (Buffalo) R. Dernick (Hamburg) V. Doln¨ªk (Mountain View) N. J. Dovichi (Seattle) M. J. Dunn (London) D. Ehrlich (Cambridge) S. Fanali (Roma) B. Gas¡¦ (Prague) E. Gianazza (Milano) H. H. Girault (Lausanne) A. Görg (Freising) A. Guttman (San Diego) P. R. Haddad (Hobart) J. H. Hahn (Pohang) N. Heegaard (Copenhagen) T. Hirokawa (Higashi-Hiroshima) S. Honda (Osaka) Cs. Horvath (New Haven) L. A. Huber (Innsbruck) H. J. Issaq (Frederick) K. Jinno (Toyohashi) B. L. Karger (Boston) V. Kas¡¦ ic¡¦ ka (Prague) E. Kenndler (Wien) T. Kitamori (Tokyo) J. Klose (Berlin) L. Kr¡¦iv¨¢nkov¨¢ (Brno) S. F. Y. Li (Singapore) B. Lin (Dalian) S. M. Lunte (Lawrence) R. A. Mathies (Berkeley) K. Nakamura (Ube) V. Neuhoff (Göttingen) S. Nilsson (Lund) V. Pallini (Siena) C. P. Palmer (Missoula) W. F. Patton (Eugene) A. Paulus (Sunnyvale) T. Rabilloud (Grenoble) V. T. Remcho (Corvallis) M.-L. Riekkola (Helsinki) P. G. Righetti (Verona) A. M. Rizzi (Wien) V. Schurig (T¨¹bingen) G. Scriba (Jena) S. Shoji (Tokyo) G. W. Slater (Ontario) R. D. Smith (Richland) F. Svec (Berkley) J. Sweedler (Urbana) K. Taketa (Ibara) W. Thormann (Bern) E. Verpoorte (Neuchâtel) O. Vesterberg (Stockholm) G. Vigh (College Station) J. L. Viovy (Paris) H. Wätzig (Braunschweig) G. M. Whitesides (Cambridge) E. S. Yeung (Ames) Editorial Assistant M. Frey-Goerth (E-mail: manuela.d.frey@t-online.de, Fax: +49-9931-5395) For USA and Canada: Electrophoresis (ISSN 0173-0835) publishes 24 issues in 2003. Air freight and mailing in the USA by Publications Expediting Inc., 200 Meacham Ave., Elmont, NY 11003. Periodicals paid at Jamaica, NY 11431. Annual full subscription price in 2003 US $ 3008.00 (print or online) or US $ 3159.00 (print & online), includes the journal Proteomics. Rates for Electrophoresis only and for personal subscription available. Prices include handling and postage. Printed in the Federal Republic of Germany. U.S. Postmaster: Send address changes to: Electrophoresis, c/o Wiley-VCH, 111 River Street, Hoboken, NJ, 07030. Publisher WILEY-VCH Verlag GmbH & Co. KGaA P.O. Box 101161 D-69451Weinheim (Germany) Telephone +49-6201-606-0 Telefax +49-6201-606-207 Journal Management, Advertising H.-J. Kraus (E-mail: hkraus@wiley-vch.de) Marketing V. Bluteau (E-mail: vbluteau@wiley-vch.de) Production, Reprints B. Hill (E-mail: bhill@wiley-vch.de) Copyright Permissions C. Rutz (E-mail: crutz@wiley-vch.de) Language Polishing Authors in Japan please note: Wiley-Japan can provide authors in Japan with a list of recommended services to check and improve the English of their papers before submission. Please contact A. Bocquet in the Wiley-Japan office by E-mail (wileyjpn@mb.kcom.ne.jp) or Fax (+81-3-3556-9763) for more information. Subscription Service For customers in Germany, Austria and Switzerland: WILEY-VCH, Journals Administration Department, P.O. Box 101161, D-69451Weinheim, Germany E-mail: subservice@wiley-vch.de Fax: +49-6201-606-172 Phone: +49-6201-606-147 For customers in all other countries: John Wiley & Sons Ltd., Journals Administration Department, 1 Oldlands Way, Bognor Regis, West Sussex PO22 9SA, England E-mail: cs-journals@wiley.co.uk Fax: +44-1243-843-232 Phone: +44-1243-779-777 Homepage http://www.wiley-vch.de/home/electrophoresis Instructions to Authors Please refer to the homepage of the journal at the URL as given above. Printed on acid-free paper 2003 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. Printed in the Federal Republic of Germany. Editor-in-Chief: Prof. B. J. Radola, Technische Universität M¨¹nchen, D-85350 Freising-Weihenstephan. Publisher:WILEY-VCH Verlag GmbH & Co. KGaA, Boschstraße 12, D-69469 Weinheim, Federal Republic of Germany. Correspondence concerning advertisements should be addressed to WILEY-VCH Verlag GmbH & Co. KGaA, Boschstraße 12, D-69469 Weinheim, Telephone (06201) 606-311, Telefax (06201) 606-202, E-mail: sales journals @ VCHGROUP.DE. Institutional subscription rates 2003 (print or online/ print & online: Europe 2928.00/3075.00, Switzerland sFr 5178.00/5437.00, Outside Europe US $ 3008.00/ 3159.00. Institutional subscription includes the journal- Proteomics. Prices include postage and handling. Rates for Electrophoresis only and for personal subscribers available. Prices are subject to change. Rate of publication: 24 times a year (2003). Cancellation of subscriptions: The publisher must be notified not later than three months before the end of the calendar year. Books for review: Uninvited copies not chosen for review will not be returned. Printed by Rheinhessische Druckwerkstätte, Alzey All rights reserved (including those of translation into other languages). 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